URINE CULTURE

The quantification of bacteria in urine can be done by several methods. Among them sterile filter paper strip method, calibrated loop technique and multipoint technology are the easiest and routinely used methods (Fallon et. al., 2003) (Haugen et. al., 1968). Of these, multipoint methodology using CLED or chromogenic media, are considered to be the most versatile and efficient for large numbers of specimens. Other methods include use of dipslides, pour plates and roll tubes. (Aspevall et. al., 2002)

Filter paper method

1.      Commercially prepared sterile filter paper strip was dipped in the urine up to the mark indicated. .

2.      Excess or the urine was removing by touching the edge of the strip against the side of the specimen container.

3.      The remaining urine was allowed to absorb into the strip before inoculating a CLED or chromogenic agar plate.

4.      Inoculated end of the strip was Bend and press flat against the agar for a few seconds.

5.      Several specimens were inoculated onto one CLED agar plate (Fallon et. al., 2003).

Figures1,2

Antimicrobial Senstivity:

The Kirby-Bauer (K-B) test utilizes small filter disks impregnated with a known concentration of antibiotic. The disks are placed on a Mueller-Hinton agar plate that is inoculated with the test microorganism. Upon incubation, antibiotic diffuses from the disk into the surrounding agar. If susceptible to the antibiotic, the test organism will be unable to grow in the area immediately surrounding the disk, displaying a zone of inhibition. The size of this zone is dependent on a number of factors, including the sensitivity of the microbe to the antibiotic, the rate of diffusion of the antibiotic through the agar, and the depth of the agar. Microorganisms that are resistant to an antibiotic will not show a zone of inhibition, (accessed 5-12-17)

Shows the Filter paper Urine culture strip

Shows the Bottle of filter paper urine culture strip