Principle:
Many organisms especially which
infect the urinary tract are able to split urea into carbon dioxide and ammonia
in the presence of water. Ammonia combines with both water and carbon dioxide
and produces the ammonium carbonate. This ammonium carbonate changes the pH of
medium to alkaline and produces a pink color from original yellow color.Accessed:16-12-16
Procedure
1-Pour the pure bacteria suspension
into the broth medium.
2-Loosely tight the cap of tube and
leave at 35 °C ambient air.
3-Observe
the result. Accessed:16-12-16
Result
No change
considered as negative result. Figure
3
Color
change into pink will be indicated the result as positive test. Figure 4
Accessed:16-12-16
Shows Negative
Urease test
Shows Positive
urease test
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