E.Coli is normally present in the
intestinal tract of humans and animals but they are also present in the water
and soil. They gram negative bacteria present in the form of rods. They are mostly
motile except some strains. E.coli is
non spore forming bacteria. (Monica2000)
1.3.1:Cultural
Characteristics include:
1)
Facultative
Anaerobes
2) Optimum
temperature for growth :
i.
35 to 37°C.
3)
On
blood agar:
i.
1-4 mm calonies
, round , having entire edge.
4)
Some
strains hemolytic
5)
On
MacConkey agar :
i.
Ferment lactose
ii. Pink
calonies(Figure1)
iii. Some
strains non motile and non lactose fermentors.
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