TRIPLE SUGAR IRON

Procedure

1- with the help of a sterilized wire loop pick up a well isolated colony from medium.

2- stab the wire in the center of TSI medium .

3- then inoculate it bt making a zigzag position with wire loop on the slant of agar.

4- incubate the tube at 37C for 24 hours.

5- observe the results carefully. Accessed:19-12-16

Results:

If the lactose ferment by the organism present then butt and slant of medium turned yellow.

If the lactose not fermented then butt is turned into yellow while slant becomes red.

If the glucose lactose and sucrose not fermented both butt and slant turned red. Accessed:19-12-16

See the figures

Shows positive TSI test

Shows positive TSI test