Initial
examination:
The appearance
of a specimen of pus and that of any appreciable amount of pus on a swab was
observed (Koneman et. al., 2005).
Characterization
of Bacterial Isolates:
1. The
pus specimen was inoculated on blood and MacConkey agar plates.
2. The
streaked plates were incubated at 37°C for 24 hr.
3. Bacterial
colonies on blood agar plates were later Gram stained.
4. Characterization
of bacterial isolates was based on standard microbiological methods.
5. Identification
of isolates were done based on colony morphology, motility, catalase test,
oxidase test, coagulase test and biochemical tests like Tripal sugar iron agar,
Hydrogen sulfide test, Carbohydrate fermentation test, Phenylalanine deaminase
test, Methyl red test, Nitrate reduction test, Urease test, Voges proskauer,
Citrate utilization test, Indole test (Koneman et. al., 2005).
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